The MIQE guidelines and papers such as the one discussed here can be applied to avoid pitfalls. Reverse-transcription quantitative polymerase chain reaction (RT-qPCR) is everywhere. Its ubiquity ...

Normalization of qPCR data Watch now! Comparison of expression data requires normalization. The optimum normalization method depends on sample type, with the most common being to normalize to ...

Gene profiling using quantitative PCR is becoming higher throughput, but researchers must be careful in gathering their data.

In total, Biogazelle performed more than 80,000 qPCR reactions. These reference data matched well with RNA-seq results. “Our motivation to participate in the SEQC study was to add a valuable reference ...